Klebsiella pneumoniae biochemical identification kit|BNCC361613 |BNCC

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Klebsiella pneumoniae biochemical identification kit

Safety level	1
application	Biochemical identification of microorganisms, biochemical identification of Klebsiella pneumoniae.
Sharing mode	Public welfare sharing

Klebsiella pneumoniae biochemical identification kit

1. Product details

product name	Klebsiella pneumoniae biochemical identification kit
number	BNCC 361613		batch	220530
store	2-8℃		validity period	1 year
reaction description	there are 17 pieces of this product, as follows:
	serial number	reagent name	description	judge color
				positive +	negative-
	1	MR-VP(1)	MR test	red	unchanged
		methyl red indicator (1)
	2	MR-VP(1)	VP test	red	unchanged
		VP reagent (2)
	3	nitrate broth (1)	nitrate reduction test	red	unchanged
		nitrate reduction kit (2)
	4	peptone water (1)	indigo matrix test	red ring	yellow or brown
		Kovacs indigo matrix reagent (1)
	5	malonate medium (1 branch)	malonate utilization test	turn blue	unchanged
	6	urease medium (1 branch)	urease test	change rose red	unchanged or yellow
	7	lysine decarboxylase medium (1 branch)	lysine decarboxylase test	purple	yellow
	8	ornithine decarboxylase medium (1 branch)	ornithine decarboxylase test	purple	yellow
	9	lactose fermentation tube (1)	lactose fermentation test	yellow	purple
	10	Simon's citrate medium (1)	citrate utilization test	blue	green
	11	glucose fermentation tube (1	glucose fermentation test	yellow	blue-green

2.Instructions

The target strains were respectively inoculated into medium 5-11, MR-VP in 1-4, nitrate broth, and peptone water, and incubated for more than 24 hours to observe the results. Among them, 1~4 need to be observed after adding the corresponding indicator dropwise. VP test: add 0.6ml of solution A dropwise and mix well with the culture, then add 0.2ml of solution B and mix well. After shaking the test tube, let it stand for 0.5~2h for observation. Nitrate reduction test: add 2-3 drops of solution A and solution B, mix well and observe.

3.Klebsiella pneumoniae discrimination criteria

number	1	2	3	4	5	6	7	8	9	10	11
reaction	-	+	+	-	+	+	+	-	+	+	+

*Note: For positive and negative colors, see the above reaction description points. If the results meet the above criteria, it can be judged as Klebsiella pneumoniae.

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