Standard quality control sample of fecal coliform (multi-tube fermentation method)|BNCC359289 |BNCC

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Standard quality control sample of fecal coliform (multi-tube fermentation method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 200mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 200mL of water sample to be tested, with a concentration of 300 ~ 3000MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	It is suitable for the determination of fecal coliforms in water quality, quality control and personnel assessment (multi-tube fermentation method). Reference Standard "HJ 347.2-2018 Water Quality Determination of Fecal Coliforms Multi-tube Fermentation Method"
Sharing mode	Public welfare sharing

Fecal coliform standard quality control sample (multitube fermentation method)

Product number: BNCC 359289

Production batch: 20210719

1. Product features:

description	The appearance is white powder (freeze-dried powder), containing protective agent, and packaged in 5mL penicillin bottle.
traceability	ATCC 25922 Escherichia coli, ATCC 13048 Enterobacter aerogenes
concentration value	300~3000MPN/L
uniformity and stability	random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	《determination of fecal coliforms in HJ347.2-2018 water quality》multi-tube fermentation method
* Note: If you have any questions about quality control samples, please contact BNCC for help before use.

2. Instructions

The sample requires a total of 200mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 200mL of water sample to be tested, with a concentration of 300 ~ 3000MPN/L.

3. Precautions

1. after receiving the goods, please keep it properly. if it is damaged, please contact the center within 24 hours after receiving it.

2. Please operate in a sterile environment and a biosafety cabinet when using. After opening, you must use it all at once.

3. this product contains three types of pathogenic microorganisms, please do not directly touch the samples in the bottle with your hands, please discard the residual samples after high temperature and high pressure sterilization.

4. Preservation and transportation

long-term storage:-20 ℃, valid for 2 years; Transportation conditions: ice bag transportation, the time should not exceed 10 days.

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