Yersinia enterocolitica nucleic acid detection kit (fluorescent PCR method)|BNCC361445 |BNCC

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Yersinia enterocolitica nucleic acid detection kit (fluorescent PCR method)

Subculture procedure	Sample processing: sample processing is carried out according to GB 4789.8-2016 National Food Safety Standard Yersinia Enterocolitica Test; Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 16 μL, template 1 μL, sterile ddH2O 8 μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 57 ℃, 30s, 40cycle; Fluorescence channel: VIC;
Safety level	1
application	It is suitable for the auxiliary diagnosis of Yersinia enterocolitica infection in water-like feces, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing

Certificate of yersinia enterocolitica nucleic acid detection kit

1. Product information

Sample name: Yersinia enterocolitica nucleic acid detection kit (fluorescent PCR method)

Sample number: 361445

Sample batch: 220505

2. Product features

Reagent composition	reaction solution 240μL/tube, sterile ddH2O 500μL/tube, positive quality control 20μL/tube, negative quality control 20μL/tube
traceability	Yersinia enterocolitica CICC21669, Escherichia coli ATCC25922
save and transport	-20 ℃, valid for 1 year, dry ice transportation
applicable fields	Suitable for auxiliary diagnosis of Shigella infection in water-like feces, suspected contaminated water, food and other samples
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instructions

Sample processing: sample processing according to "GB 4789.8-2016 National Food Safety Standard - Inspection of Yersinia enterocolitica";

Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

Reagent preparation: experimental reaction system (25 μL), 16 μL qPCR mixed reaction solution, 1 μL template, 8 μL sterile ddH2O

Reaction conditions: 95℃ 1min 1cycle; 95℃ 10s, 57℃ 30s 40cycle;

Fluorescence channel: VIC;

4. Notes

Negative: The sample has no exponential growth curve, or has a lower amplification curve, and the Ct value is >38;

Positive: the sample has a typical exponential growth curve, and the Ct value is ≤38;

5. Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

Website: www.bncc .org .cn Tel: 400-6699-833

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