Listeria monocytogenes nucleic acid detection kit (fluorescent PCR method)|BNCC361444 |BNCC

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Listeria monocytogenes nucleic acid detection kit (fluorescent PCR method)

Subculture procedure	Sample processing: sample processing is carried out according to GB 4789.30-2016 National Food Safety Standard for Listeria monocytogenes Detection; Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15 μL, template 1 μL, sterile ddH2O 9 μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 57 ℃, 30s, 40cycle; Fluorescence channel: FAM;
Safety level	0
application	It is suitable for auxiliary diagnosis of Listeria monocytogenes infection in water samples, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing

Listeria monocytogenes nucleic acid test kit certificate

1. Product information

Sample name: Listeria monocytogenes nucleic acid detection kit (fluorescent PCR method)

Sample number: 361444

Sample batch: 220505

2. Product features

Reagent composition	reaction solution 225μL/tube, sterile ddH2O 500μL/tube, positive quality control 20μL/tube, negative quality control 20μL/tube
traceability	ATCC19115 Listeria monocytogenes and ATCC33090 harmless Listeria
save and transport	-20 ℃, valid for 1 year, dry ice transportation
applicable fields	Suitable for auxiliary diagnosis of Shigella infection in water-like feces, suspected contaminated water, food and other samples
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instructions

Sample processing: sample processing in accordance with 《GB 4789.30-2016 National Food Safety Standard - Detection of Listeria monocytogenes》;

Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

Reagent preparation: experimental reaction system (25 μL), 15 μL qPCR mixed reaction solution, 1 μL template, 9 μL sterile ddH2O

Reaction conditions: 95℃ 1min 1cycle; 95℃ 10s, 57℃ 30s 40cycles; Fluorescence channel: FAM;

4. Notes

Negative: The sample has no exponential growth curve, or has a lower amplification curve, and the Ct value is >38;

Positive: the sample has a typical exponential growth curve, and the Ct value is ≤38;

5. Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833

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