Proteus quantitative strain|BNCC356096 |BNCC

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Proteus quantitative strain

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	30 ℃, aerobic
Storage conditions	-80 ℃
morphology	Purity: pure
application	Kauffmann serotype 23ac 12a
Sharing mode	Public welfare sharing

Proteus sp.

Strain number: BNCC 356096 / CMCCB49027

Name: proteus Proteus sp.

Product format: quantitative bacteria tablets Number of viable bacteria: 5.0~6.5×10⁸CFU/bottle

Product batch: 201204 Valid until: 2021.12.03

Date of inspection: 2020.12.05 Sampling ratio: ≥2%

Report number: RS20356096-1 Biosafety level: level 2, handle in safety cabinet

project	description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli, in a single rod shape
colony morphology	observation of single colony morphology	milky white, raised, bright, or spread
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	5.7×10⁸CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	qualified

Product list: 5.0~6.5×10⁸CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Shelf life: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. after full dissolution, it is 5.0~6.5×10⁸CFU/mL, which can be sucked in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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