Campylobacter jejuni (CJ) nucleic acid detection kit (fluorescent PCR method)|BNCC360690 |BNCC

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Campylobacter jejuni (CJ) nucleic acid detection kit (fluorescent PCR method)

Subculture procedure	1. Experimental preparation: dissolve various components in the kit on ice before use, mix slightly and centrifuge briefly, and then place them on ice for later use; 2. Sample processing: sample processing shall be carried out according to SN/Campylobacter T3639-2013 jejuni test; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 21 μL, template 4 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 50 ℃ 30s 40cycle; 5. Fluorescence channel: FAM;
Storage conditions	-20 ℃; Keep away from light
Safety level	1
application	It is suitable for auxiliary diagnosis of Campylobacter jejunum (CJ) infection in water-like feces, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing

Campylobacter jejuni nucleic acid test kit certificate

1. Product information

Sample name: Campylobacter jejuni (CJ) nucleic acid detection kit (fluorescent PCR method)

Sample number: 360690

Sample batch: 220401

2. Product features

Reagent composition	reaction solution 525μL/tube, positive quality control 100μL/tube, negative quality control 100μL/tube
traceability	Campylobacter ATCC33291 jejuni (CJ), Campylobacter Colon ATCC43478
save and transport	-20 ℃, valid for 1 year, dry ice transportation
applicable fields	It is suitable for auxiliary diagnosis of Campylobacter jejunum infection in water-like feces, suspected contaminated water, food and other samples
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Use steps

1. Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

2. Sample processing: sample processing according to "SN/T3639-2013 Campylobacter jejuni test";

3. Reagent preparation: experimental reaction system (25 μL), 21 μL qPCR mixed reaction solution, 4 μL template;

4. Reaction conditions: 95°C 3min 1cycle; 94°C 10s, 50°C 30s 40cycle;

5.Fluorescence channel: FAM;

4.Result judgment

1. The sample has a typical amplification curve, and the Ct value is less than or equal to 35;

2. The sample has no amplification curve, and the Ct value is ≥40 or not detected;

3. If the t value of the sample is >35 and <40, it needs to be re-measured. Retest results Ct value ≥ 40 is negative, Ct value < 40 is positive.

5. Storage and transport

1. The various components in the kit were dissolved on ice before use, completely mixed and centrifuged briefly, and then placed on ice for later use;

2.In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3.Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

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