Bacillus subtilis subsp nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361580 |BNCC

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Bacillus subtilis subsp nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	1
application	This product is a Bacillus subtilis nucleic acid reference (heat inactivated) (strong positive), which can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.
Sharing mode	Public welfare sharing

Bacillus subtilis nucleic acid reference certificate

1. Product information

Sample name: Bacillus subtilis nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361580

Sample batch: 220518

2. Product features

description	1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, in liquid state
traceability	Bacillus subtilis ATCC6633
preparation method	inoculation of nutrient broth medium, PBS suspension, 90 ℃ water bath inactivation for 1.5h
concentration standard	≥1.0×10⁸CFU/mL	result	4.9×10⁸CFU/mL
authentication	colony morphology is consistent with Bacillus subtilis	result	match
*Note: If you have any questions about the reference materials, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be treated according to infectious substances. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage: -80℃, valid for 2 years; transport conditions: transport on dry ice.

Henan Engineering Research Center of Industrial Microbiology

Website: www.bncc .org .cn Tel: 400-6699-833

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