Citrobacter freundii nucleic acid reference material (Heat inactivated)(Strongly positive)|BNCC360676 |BNCC

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Citrobacter freundii nucleic acid reference material (Heat inactivated)(Strongly positive)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃;18-24h; Aerobic gas environment;
Storage conditions	-80 ℃, kept away from light
Safety level	1
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product is a heat-inactivated strong positive reference for the evaluation of Citrobacter freundii nucleic acid detection reagent for early diagnosis of infection And quality control.
Sharing mode	Public welfare sharing

Certificate of Citrobacter freundii nucleic acid reference product

1. Product information

Sample name: Citrobacter freundii nucleic acid reference (heat inactivated)

Sample number: 360676

sample batch: 220208

2. Product features

Description	1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, is transparent liquid
Traceability	ATCC 43864 Citrobacter freund
Concentration value	≥10⁶CFU/mL
Applicable method	Related research on nucleic acid detection reagents for pathogens
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be treated according to infectious substances. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5.Preservation and transportation

Long-term storage:-20 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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