Quantitative strain of Clostridium sporogenes|BNCC359610 |BNCC

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Clostridium sporogenes

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Anaerobic;
Growth characteristics	G +, cell straight rod-shaped, 0.73 × 1.46~3.33 μm. Mostly a single arrangement. The spores are oval, subterminal, and the spores are enlarged. After the formation of spores, the bacteria quickly decomposed, leaving only spores.
Storage conditions	-20 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, white on the front, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), bacilli, purity: pure
application	Research; Analytical testing. Teaching and research. "Technical Specifications for Disinfection" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Therapeutics, "GB/T 4789.12-2003 Clostridium botulinum and Botulinum Toxin Test" Positive Control Strain, Quality Control Strain of Chinese Pharmacopoeia, 2015 Edition. 2020 Edition Pharmacopoeia-"General Principles 1100 Biological Inspection Method" for tested strains of bacteria in pharmacopoeia, medium "sensitivity inspection" for tested strains, "method applicability test" for tested strains, "medium applicability test and control bacteria test method applicability test" for tested strains
Sharing mode	Public welfare sharing

Clostridium sporogenes

Strain number: BNCC 359610 /CMCCB 64941

Product format: quantitative bacteria tablets

Product batch:220309

Date of inspection:22022.03.10

Valid until:2024.03.08

Number of viable bacteria:50-150CFU/bottle

Sampling ratio:≥2%

Report number:RS22359610-1

Biosafety level: 2 handle in biosafety cabinet

Items	test results	description
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + bacilli
colony morphology	observation of single colony morphology	light yellow, moist, raised, with neat edges
purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	63CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
Conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list: 1 bottle of 50-150CFU quantitative strain, 1 bottle of 1mL lysate, 1 strain certificate;

Validity period: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is 50- 150CFU/mL, which is absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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