Clostridium sporogenes|BNCC360712 |BNCC

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Clostridium sporogenes

Culture medium	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat and stomach enzyme digest 5.0g, yeast leaching powder 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3 0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h; Anaerobic
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Off-white Protuberance: Flat Surface: Grey Dark Texture: Wet
Sharing mode	Public welfare sharing

Clostridium sporogenes

Storage conditions: 2~8 ℃

No. 360712

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)The plate is cultured under the above-mentioned culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 48h strain layer become obvious, colony is typical
colony morphology:	size: 2-4mm shape: irregular edge: irregular transparency: opaque color: off-white uplift: flat surface: gray texture: moist
Conclusion:	colony morphology is not abnormal, qualified

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