Quantitative strain of Escherichia coli|BNCC353742 |BNCC

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Quantitative strain of Escherichia coli

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	37 ℃, aerobic
Storage conditions	-20 ℃
morphology	Pale yellow, raised, moist, bright, G-bacilli
application	Research
Sharing mode	Public welfare sharing

Escherichia coli

No. : 353742 / ATCC 8739

Product format : quantitative bacteria tablets

Number of live bacteria : 3.5~5.0×10 3CFU/bottle

Product batch : 191203

Valid until : 2020.6.2

Date of inspection : 2019.12.4

Sampling ratio :≥2%

Report number : RS19353742-1

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

item	description	test results
stain microscopy	gram-negative and positive, cell morphology observed under microscope	G + cocci, in a single rod shape
colony morphology	observation of single colony morphology	milky white, raised, bright, or spread
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	4300CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :3.5~5.0 & times;10 3CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period : unopened, below -20 ℃, 6 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 3.5~5.0×10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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