BeNa Culture Collection

Quantitative strain of Escherichia coli-BNCC
Quantitative strain of Escherichia coli-BNCC
Quantitative strain of Escherichia coli-BNCC
  • BNCC
  • Quantitative strain of Escherichia coli-BNCC
  • Quantitative strain of Escherichia coli-BNCC
  • Quantitative strain of Escherichia coli-BNCC

Quantitative strain of Escherichia coli

  • Price: $115
  • number:BNCC353742
  • Packing:penicillin bottle;(3.6±0.6)× 10 3CFU/bottle
  • Form:
    Pale yellow, raised, moist, bright, G-bacilli
Essential Information Certificate Related Products
Quantitative strain of Escherichia coli
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions 37 ℃, aerobic
Storage conditions -20 ℃
morphology Pale yellow, raised, moist, bright, G-bacilli
application Research
Sharing mode Public welfare sharing

Escherichia coli

No. : 353742 / ATCC 8739

Product format : quantitative bacteria tablets

Number of live bacteria : 3.5~5.0×10 3CFU/bottle

Product batch : 191203

Valid until : 2020.6.2

Date of inspection : 2019.12.4

Sampling ratio :≥2%

Report number : RS19353742-1

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

item description test results
stain microscopy gram-negative and positive, cell morphology observed under microscope G + cocci, in a single rod shape
colony morphology observation of single colony morphology milky white, raised, bright, or spread
purity non-selective medium culture, colony observation and microscopic examination No bacteria
Reference value of live bacteria non-selective medium plate, live bacteria count 4300CFU/bottle
phenotype and genotype analysis phenotypic testing, gene sequencing qualified
test conclusion after quality inspection by BNCC quality control testing system, the product is qualified  

Product list :3.5~5.0 & times;10 3CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period : unopened, below -20 ℃, 6 months;

Handling procedure:

1  Open the penicillin-bottle according to the arrow indication on the vial cap;
2  Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3  It will be 3.5~5.0×10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4  The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2  Waste generated from the handling process should be discarded after high-pressure sterilization

 

 

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