Promicromonospora sukumoe|BNCC360731 |BNCC

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Promicromonospora sukumoe

Culture medium	R2A agar medium (English name: R2A): tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast powder 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH 7.2±0.2. 121 ℃,15min sterilization
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;24-48h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm Shape: Round Edge: Irregular Transparency: Opaque Color: Yellow Uplift: Flat Surface: Rough Fold Texture: Dry, G-(Red), Bacillus
Separation substrate	soil
Sharing mode	Public welfare sharing

Promicromonospora sukumoe

Storage conditions: 2~8 ℃

No. 360731

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, R2A medium, 24-48h. R2A medium: tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3g, agar 12g, peptone 0.25g g, glucose 0.5g, pH7.2±0.2. Sterilize at 121℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 40h plate bacteria layer is obvious; colony is typical on the streaked plate
colony morphology:	Size: 0.5-1mm Shape: Round Edge: Irregular Transparency: Opaque Color: Yellow Protuberance: Flat Surface: Rough Fold Texture: Dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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