Saccharopolyspora sp.|BNCC360152 |BNCC

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Saccharopolyspora sp.

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;2-3 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: Light Yellow Protuberance: Middle convex Surface: Rough Gray Texture: Dry and Hard to Pick
Separation substrate	plant sample
Sharing mode	Public welfare sharing

Saccharopolyspora sp.

Storage conditions : 2~8 ℃

No. : 360152

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, Streptomyces medium No. 2. 2-3 days, Streptomyces medium No. 2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2 ±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) The plate was cultured under the above-mentioned culture conditions, and the bacteria were grown and used.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 3 dyas,strain layer is obvious;colony is typical on the streaked plate
colony morphology:	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: light yellow uplift: middle raised surface: rough gray texture: dry and difficult to pick
Conclusion:	good viability, no abnormal colony morphology, qualified

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