BeNa Culture Collection

Klebsiella pneumoniae subsp. rhinoscleromatis-BNCC
Klebsiella pneumoniae subsp. rhinoscleromatis-BNCC
  • BNCC
  • Klebsiella pneumoniae subsp. rhinoscleromatis-BNCC
  • Klebsiella pneumoniae subsp. rhinoscleromatis-BNCC

Klebsiella pneumoniae subsp. rhinoscleromatis

  • Price: $ 172
  • number:BNCC341952
  • Form:
    Size: 2-4mm Shape: Round Edge: Neat Transparency: Translucent Color: off-white Protuberance: Middle Protuberance Surface: Smooth and Bright Texture: Sticky and moist
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Klebsiella pneumoniae subsp. rhinoscleromatis
Culture medium Tryptone soybean medium (TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 2-4mm Shape: Round Edge: Neat Transparency: Translucent Color: off-white Protuberance: Middle Protuberance Surface: Smooth and Bright Texture: Sticky and moist
Sharing mode Public welfare sharing

Klebsiella pneumoniae subsp

Storage conditions : 2~8 ℃

No. : 341952

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :37 ℃, aerobic, tryptone soybean medium, 18-24h. Tryptone soybean medium: tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                           

item test results
viability good viability, in 20h liquid medium become turbid,  obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology: (above) size: 2-4mm shape: round edge: neat transparency: translucent color: off-white uplift: middle raised surface: smooth and bright texture: viscous and moist
conclusion good viability, no abnormal colony morphology, qualified
Download certificate
Please set your password: