Sarocladium sp.|BNCC341082 |BNCC

Essential Information Certificate Related Products

Sarocladium sp.

Culture medium	Comprehensive PDA + lactose (CPDA + lactose): comprehensive PDA + lactose (1 liter of common PDA plus 5g lactose): 1000mL of potato boiled juice, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate, 20g of glucose, 10mg of vitamin B1, 20g of agar, and natural pH. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies, white, dense and vigorous hyphae on the comprehensive PDA + lactose medium, spreading and growing to the edge of the plate, and the back of the medium was light yellow.
Sharing mode	Public welfare sharing

Description

1. Name: Sarocladium sp.

2. BNCC No.: 341082

3.Biosafety level: 4

Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. Comprehensive PDA + lactose (1 liter of common PDA plus 5g lactose): potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2.Atmosphere: aerobic

3. Temperature: 22 ℃

Notes:
1.Normal culturing time, 1-2 days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate