Aspergillus oryzae (Ahlburg) Cohn|BNCC338380 |BNCC

Aspergillus oryzae (Ahlburg) Cohn

Price： $ 86
number：BNCC338380
Form：
Small filamentous fungi, with obvious colonies, white, dense and vigorous hyphae on the integrated PDA medium, later producing yellow-green spores, spreading and growing to the edge of the plate, and the back of the medium was yellow.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Aspergillus oryzae (Ahlburg) Cohn

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies, white, dense and vigorous hyphae on the integrated PDA medium, later producing yellow-green spores, spreading and growing to the edge of the plate, and the back of the medium was yellow.
application	Research; Production. Production of saccharifying enzyme, amylase, alcohol production of saccharifying bacteria.
Sharing mode	Public welfare sharing

Description

1. Name:Aspergillus oryzae (Ahlburg) Cohn

2. BNCC No.: 338380

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1, Czapek ' Sagar: sucrose 30.0g,NNaNO3 3.0 g，MgSO4·7H2O 0.5 g，KCl 0.5g，FeSO4·4H2O 0.01 g，K2HPO4 1.0 g, agar 15.0g, distilled water 1.0 L,pH 6.0-6.5,121 ℃,15min.

2. Atmosphere:aerobic

3, Temperature: 25-28 ℃

Notes:

1.Normal culturing time, 1-2 days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization

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