BeNa Culture Collection

Aspergillus oryzae (Ahlburg) Cohn-BNCC
  • BNCC
  • Aspergillus oryzae (Ahlburg) Cohn-BNCC

Aspergillus oryzae (Ahlburg) Cohn

  • Price: $ 115
  • number:BNCC336577
  • Form:
    Small filamentous fungi, with obvious colonies on malt extract medium, white hyphae, yellow-green spores in the later period, raised in the center of the colony and no spores, and brown on the back of the medium.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Aspergillus oryzae (Ahlburg) Cohn
Culture medium Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on malt extract medium, white hyphae, yellow-green spores in the later period, raised in the center of the colony and no spores, and brown on the back of the medium.
application Brewing soy sauce.
Sharing mode Public welfare sharing

1.Description

1. Name: Aspergillus oryzae (Ahlburg) Cohn

2. BNCC No.:336577

3. Biosafety level : 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, 5 Be′ Wort agar: 5 Be Wort 1.0L, natural pH.

2. Atmosphere:aerobic

3, Temperature: 28-30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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