BeNa Culture Collection

Aureobasidium pullulans-BNCC
Aureobasidium pullulans-BNCC
Aureobasidium pullulans-BNCC
  • BNCC
  • Aureobasidium pullulans-BNCC
  • Aureobasidium pullulans-BNCC
  • Aureobasidium pullulans-BNCC

Aureobasidium pullulans

  • Price: $ 86
  • number:BNCC335955
  • Form:
    Colony diameter is 6-8mm, irregular shape, irregular edge, opaque, dark color, dark color, flat shape, purity: pure, G (blue-purple), cocci, firm and hard texture, difficult to pick/cut
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Aureobasidium pullulans
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate μL/piece, and spread it evenly.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
morphology Colony diameter is 6-8mm, irregular shape, irregular edge, opaque, dark color, dark color, flat shape, purity: pure, G (blue-purple), cocci, firm and hard texture, difficult to pick/cut
Sharing mode Public welfare sharing

1.Description

1. Name :  Aureobasidiumpullulans(deBary)Arnaud

2, BNCC No.: 335955 

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃

4. Notes: 
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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