Streptomyces rimosus Sobin et al.|BNCC335932 |BNCC

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Streptomyces rimosus Sobin et al.

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;2-3 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: round Edge: Irregular Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: fold Texture: Dry
application	Oxytetracycline production
Sharing mode	Public welfare sharing

1.Description

1. Name: Streptomyces rimosus Sobin et al.

2. BNCC No.: 335932

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Potato and glucose agar: take 200 grams of peeled potato, cut it into small pieces, add 1000 milliliters of water to boil for 30 minutes, filter out potato pieces, fill up the filtrate to 1000 milliliters, add 20 grams of glucose and 15 grams of agar, dissolve and pack it, sterilize 15 pounds for 30 minutes.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4. Notes:
1.Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization

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