Aspergillus parasiticus Speare, anamorph|BNCC263916 |BNCC

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Aspergillus parasiticus Speare, anamorph

Culture medium	Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃,3-5 days, aerobic
Storage conditions	2-8°C
morphology	Small filamentous fungi, with obvious colonies on malt extract medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, producing white spores, and the back of the medium is orange-yellow.
Separation substrate	UV mutant of Aspergillus parasiticus NRRL A- 16462
application	produces norsolorinic acid dehydrogenase;produces versicolorin A
Sharing mode	Public welfare sharing

Aspergillus parasiticus Speare, anamorph

Storage conditions : 2~8 ℃

No. 263916

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:24 ℃, aerobic, malt extract medium, 3-5 days. Malt extract medium: malt extract 20.0g, glucose 20.0g (added before sterilization), peptone 1.0g, agar 20.0g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 3 days strain layer is obvious
colony morphology:	Small filamentous fungi with obvious colonies on malt extract medium, White, dense and vigorous hyphae, spreading to the edge of the plate, orange on the back of the medium
Conclusion:	good viability, no abnormal colony morphology, qualified ;

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