Microsporum canis|BNCC259680 |BNCC

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Microsporum canis

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 5-7 days.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
morphology	Spread growth, gray-white spores, gray-white front, dark
Separation substrate	Scalp ringworm
application	Biomedical Research and Development Material
Sharing mode	Public welfare sharing

Microsporum canis Bodin anamorph

Storage conditions: 2~8 ℃

No. 259680

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Sterilize the plate surface, open it in biosafety cabinet；

(3) Pick up 1-2 rings of plate colonies by inoculation loop, inoculate to fresh medium；

(4) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 7 days, plate colony is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies on the integrated PDA medium. The mycelium in the plate layer is white and dense, spreading and growing, producing gray-white spores in the later stage, and the back of the medium is yellow.
Conclusion	good viability, no abnormal colony morphology, qualified

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