Bifidobacterium subsp.|BNCC256597 |BNCC

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Bifidobacterium subsp.

Culture medium	Enhanced Clostridium agar medium (RCM): yeast extract 3.0g, beef extract 10.0g, glucose 5.0g, tryptone 10.0g, sodium chloride 5.0g, soluble starch 1.0g, cystine hydrochloride 0.5g, sodium acetate 3.0g, agar 20g,pH 6.8±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,24-48h, anaerobic 80% nitrogen-10% carbon dioxide-10% hydrogen
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Irregular Edge: Neat Transparency: Opaque Color: milky white uplift: middle convex surface: bright and smooth texture: moist and viscous
Separation substrate	Chicken feces, animal
Sharing mode	Public welfare sharing

P23 Bifidobacterium animalis subsp.; lactis

Storage conditions: 2~8 ℃

No.: 256597

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, enhanced clostridium medium, 24-48h, enhanced clostridium medium: tryptone 10.0g, beef paste 10.0g, yeast powder 3.0g, glucose 5.0g, sodium chloride 5.0g, soluble starch 1.0g,L-cysteinic acid hydrochloride 0.5g, sodium acetate 3.0g, agar 20.0g (liquid medium does not contain agar, and add 4ml blade azure), distilled water 1.0l. pH 6.8±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 48h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology (above)	size: 1-2mm shape: irregular edge: neat transparency: opaque color: milky white uplift: middle raised surface: bright and smooth texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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