Enterococcus faecalis|BNCC192631 |BNCC

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Enterococcus faecalis

Culture medium	Bifidobacterium culture medium (BM): soybean peptone 5.0g, tryptone 5.0g, yeast extract 10.0g, glucose 10.1g, salt solution 40.0 ml,L-cysteine 0.5g,0.1% azure 1.0 ml, agar 15.0g, distilled water 1.0L,pH 7.0. Salt solution: CaCl2 0.2g,MgSO4 · 7H2O 0.48g,K2HPO4 1.0g,KH2PO4 1.0g,NaHCO3 10.0g,NaCl 1.0g, distilled water 1.0L,pH 6.5. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;48-72h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: milky white bulge: middle bulge surface: bright and smooth texture: moist and viscous
Separation substrate	Yogurt
application	Fermented pickles, fruit and vegetable products, feed, dairy products, etc.
Sharing mode	Public welfare sharing

Enterococcus faecalis

storage conditions: 2~8 ℃

No. : 192631

product format： freeze dried, 200ul

Validity: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:37 ℃, aerobic, bifid enrichment medium, 48-72h. Bifid enrichment medium: peptone 15.0g, glucose 20.0g, yeast extract 2.0g, soluble starch 0.5g, sodium chloride 5.0g,L-cysteine 0.5g, tomato powder 5.0g, liver powder 2.0g, Tween 80 1mL, agar 20.0g (liquid medium does not contain), distilled water 1.0L,pH value 7.0 & plusmn;0.1. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viabilit. in 48-72 hours, liquid medium is turbid, strain layer on plate is obvious; colony is typical on marked plate
colony morphology: (above)	size: 1mm; Shape: round ; Edge: neat; Transparency: opaque color: milky white ; Protuberance: middle bulge ; Surface: bright and smooth;Texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified

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