Enterococcus faecalis|BNCC189926 |BNCC

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Enterococcus faecalis

Culture medium	MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;3 days; Aerobic
Storage conditions	2-8 ℃
Separation substrate	Kefir grain
application	Lactic acid starter
Sharing mode	Public welfare sharing

Enterococcus faecalis

Storage conditions: 2~8 ℃

No.: 189926

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1

Receiving notice： if any abnormal situation such as damage is found on the day of receipt, please contact customer service within 24 hours. Dry powder cannot be retained once used up. please refer to the attached page for details of the use of bacterial liquid. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:37 ℃, aerobic, 3d, MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0l. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:① prepare a test tube containing 5~10mL of liquid culture medium; (2) Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers; (3) Absorb 0.5mL of liquid culture medium into a freeze-drying tube, fully dissolve and return to the liquid test tube, and mix well; ④ Place the liquid test tube under the above culture conditions, and the strain can be used when it grows out.

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