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BNCC > Microbial strain > Trichoderma reesei
Trichoderma reesei-BNCC
Trichoderma reesei-BNCC
Trichoderma reesei-BNCC
  • BNCC
  • Trichoderma reesei-BNCC
  • Trichoderma reesei-BNCC
  • Trichoderma reesei-BNCC

Trichoderma reesei

  • Price： $ 143
  • number：BNCC191536
  • Form：
    Small filamentous fungi have obvious colonies on the comprehensive PDA medium, and the hyphae of the plate layer are white in the early stage and gradually become yellow, Yellow spores are produced in the later stage, and the back of the medium is yellow.
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Trichoderma reesei
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;3-5 days; Aerobic
Storage conditions 2-8 ℃
morphology Small filamentous fungi have obvious colonies on the comprehensive PDA medium, and the hyphae of the plate layer are white in the early stage and gradually become yellow, Yellow spores are produced in the later stage, and the back of the medium is yellow.
application Cellulase production by fermentation
Sharing mode Public welfare sharing

Trichoderma reesei

 

BNCC No.:91536

Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions: 

1. Synthesis PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural.

2. Atmosphere: aerobic (producing green spores)

3. Temperature: 28℃

Notes: 

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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