Bacillus amyloliquefaciens subspecies|BNCC188024 |BNCC

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Bacillus amyloliquefaciens subspecies

Culture medium	Glucose nutrition agar -2(G-NA-2): glucose nutrition agar -2: beef paste 10.0g, peptone 10.0g, glucose 10.0g,NaCl 5.0g, agar 15.0~20.0g, distilled water 1.0L,pH7.0. 121 ℃,15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;18-24h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 2-4mm shape: round edge: neat transparency: opaque Color: off-white uplift: flat surface: rough fold texture: dry
application	2,3 butanediol is produced.
Sharing mode	Public welfare sharing

1. Description

1. Name: Bacillus amyloliquefaciens subsp. amyloliquefaciens

2. BNCC No.: 188024

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1, Glucose nutrition agar -2: beef paste 10.0g, peptone 10.0g, glucose 10.0g,NaCl 5.0g, agar 15.0~20.0g, distilled water 1.0L,pH7.0. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

4. Notes:

1.Normal culturing time:24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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