BeNa Culture Collection
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1. Description
1. Name: Enterococcus faecium
2. BNCC No.: 186301
3. Biosafety level: 3
2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C.
3. Growth Conditions:
1. BHIA: 4.0g of bovine brain extract, 4.0g of bovine heart extract, 5.0g of peptone, 16.0g of casein peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, 20.0g of agar, pH7.4 ± 0.2. 121 ℃,15min.
2. Atmosphere: aerobic
3. Temperature: 37 ℃
4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.
2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.
3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.
4.Waste generated from the handling process should be discarded after high-pressure sterilization.