Aspergillus flavus|BNCC186211 |BNCC

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Aspergillus flavus

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	25 ℃
Storage conditions	2-8 ℃
application	Analysis of wood preservative compounds; detection of bacterial adhesion resistance; detection of fungal adhesion resistance; detection of antifungal resistance of airborne equipment; detection of antifungal resistance of aviation launchers; detection of antifungal resistance of automobile raw parts; detection of cork antifungal resistance of electrical insulation materials; detection of antifungal resistance of packaging materials; detection of varnish antifungal resistance; detection of wax antifungal resistance; detection of rice blast fungus S deaminase; detection of fungicides. This strain is recommended by ATCC as a strain for detection in RF31871 and does not produce aflatoxin.
Sharing mode	Public welfare sharing

1. Description

1. Name: Aspergillus flavus

2. BNCC No.: 186211

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Czapek ' S Agar): sucrose 30.0g,NaNO3 3.0g,MgSO4.H2O 0.5g,KCl 0.5g,FeSO4 .H2O 0.01g,K2HPO4 1.0g, agar 15.0g, distilled water 1.0L,pH6.0 ~ 6.5.

2. Atmosphere: aerobic

3. Temperature: 25 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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