Listeria ivanovii|BNCC185989 |BNCC

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Listeria ivanovii

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G Corynebacterium brevis, dynamic, catalase positive, β-hemolysis on the blood plate; negative nitrate reduction test, negative urease test, no fermentation rhamnose, mannitol; methyl Red reaction positive, V-P test positive, fermentation glucose, maltose, heptaside, xylose; trisugar iron test produces acid, no hydrogen sulfide; facultative anaerobic, the most suitable pH7.4 ~ 7.6.
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm shape: round edge: neat transparency: opaque color: off-white bulge: middle bulge surface: bright and smooth texture: moist and sticky
application	Research; Analytical testing. Research, quality control, "GB 4789.30-2010 Listeria monocytogenes test" positive control strains.
Sharing mode	Public welfare sharing

1. Description

1. Name: Listeria ivanovii

2. BNCC No.: 185989

3. Biosafety level: 3

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Brain heart extract agar (BHI): heart extract 5.0g, brain extract 12.5g, monthly peptone 10.0g, glucose 2.0g,NaCl 5.0g,Na2HPO4 2.5g, agar 15.0g, distilled water 1.0L,pH7.4.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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