Aspergillus oryzae|BNCC185955 |BNCC

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Aspergillus oryzae

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense and low, the hyphae are vigorously spreading and growing, and yellow spores are produced.
application	Brewing sauce, soy sauce.
Sharing mode	Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 185955

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28℃, aerobic, comprehensive PDA agar, 5-8 days, comprehensive PDA agar: potato boiling liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0± 0.2. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

① Prepare above 2 pieces of plates;

② Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well;

④ Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

⑤ Put the agar plates under the above culture conditions for cultivation，the strain can be used when it grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 5-8 days strain layer become obvious, colony is typical
colony morphology: (above)	filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense and low, the hyphae are vigorously spreading and growing, and yellow spores are produced.
Conclusion:	good viability, no abnormal colony morphology, qualified

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