Aspergillus oryzae|BNCC144195 |BNCC

Aspergillus oryzae

Price： $ 172
number：BNCC144195
Form：
Inspector/Date: Zhan Jiali 2021.05.24 Signature of Auditor: Before spore production and after spore production Culture conditions: 28 ℃, aerobic, integrated PDA,3-7 days, integrated PDA: potato cooking Liquid 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, Vitamin B1 trace, agar 20.0g,pH 6.0±0.2. 121 ℃,15min Bacteria. Potato boiling liquid: weigh 200g of peeled potato pieces and boil them in boiling water for 30min, Collect filtrate to a constant volume of 1.0L. Project test results Resurrection: Resurrectable, 3-7 days, plate colonies obvious Colony morphology: (pictured above) Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dense and vigorous, and yellow-green spores later. Plates overgrown.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Aspergillus oryzae

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Inspector/Date: Zhan Jiali 2021.05.24 Signature of Auditor: Before spore production and after spore production Culture conditions: 28 ℃, aerobic, integrated PDA,3-7 days, integrated PDA: potato cooking Liquid 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, Vitamin B1 trace, agar 20.0g,pH 6.0±0.2. 121 ℃,15min Bacteria. Potato boiling liquid: weigh 200g of peeled potato pieces and boil them in boiling water for 30min, Collect filtrate to a constant volume of 1.0L. Project test results Resurrection: Resurrectable, 3-7 days, plate colonies obvious Colony morphology: (pictured above) Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dense and vigorous, and yellow-green spores later. Plates overgrown.
application	Protease
Sharing mode	Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 144195

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, comprehensive PDA,5-7 days, comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 3-7 days strain layer become obvious
colony morphology: (above)	small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the initial hyphae are white, dense and vigorous, and the yellow-green spores are full of plates.
Conclusion:	good viability, no abnormal colony morphology, qualified

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