BeNa Culture Collection

Aspergillus oryzae-BNCC
  • BNCC
  • Aspergillus oryzae-BNCC

Aspergillus oryzae

  • Price: $ 143
  • number:BNCC185853
  • Form:
    Filamentous fungi, with obvious colonies on wort agar, vigorous spread and growth of hyphae, uneven surface, white and dense hyphae at the initial stage, and yellow-green spores at the edge at the later stage.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Aspergillus oryzae
Culture medium Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7d; Aerobic;
Growth characteristics Neutral protease production.
Storage conditions 2-8 ℃
morphology Filamentous fungi, with obvious colonies on wort agar, vigorous spread and growth of hyphae, uneven surface, white and dense hyphae at the initial stage, and yellow-green spores at the edge at the later stage.
application Research; Production. Brewing soy sauce.
Sharing mode Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 185853

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 25-28℃, aerobic, wort agar, 5-7 days. Wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L, pH natural. Sterilize at 121°C for 15min.

Recovery steps:

① Prepare above 2 pieces of plates;  

② Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well; 

④ Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

⑤ Put the agar plates under the above culture conditions for cultivation,the strain can be used when it grow out.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                                                 

Item test results
viability: good viability, in 7days strain layer become obvious
colony morphology: (above) filamentous fungi, with obvious colonies on wort agar, vigorous spread and growth of hyphae, uneven surface, white and dense hyphae at the initial stage, and yellow-green spores at the edge at the later stage.
Conclusion: good viability, no abnormal colony morphology, qualified

 

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