Aspergillus oryzae|BNCC185870 |BNCC

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Aspergillus oryzae

Culture medium	Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Growth characteristics	The colony texture is loose, at first white or yellow, that is, it becomes yellowish brown to light greenish brown. The reverse side is colorless. The conidia spike is radiate; the conidia stalk wall is very thin and rough; the apical sac is nearly spherical or flask-shaped; the conidia are pear-shaped or oval when they are young, and most of them become spherical or nearly spherical after old. Rough or nearly smooth. Can produce a large amount of kojic acid, strong protein decomposition.
Storage conditions	2-8 ℃
morphology	Filamentous fungi, the colonies are obvious on the wort agar medium. The colonies are white or yellow at first, that is, they become yellowish brown to light greenish brown., The surface is fluffy, the back is khaki yellow; smooth and slightly rough. Produce large amounts of green spores.
application	Research; Production. Soy sauce test.
Sharing mode	Public welfare sharing

Aspergillus oryzae

No.: 185870

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, 8 days, wort agar. wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

item	test results
viability:	good viability,in 8 days strain layer become obvious, colony is typical
colony morphology: (above)	filamentous fungi, the colonies are obvious on the wort agar medium, and the colonies are white or yellow at first, that is, they become yellowish brown to light greenish brown. The surface is fluffy, the back is khaki yellow; smooth and slightly rough. Produce large amounts of green spores.
conclusion:	good viability, no abnormal colony morphology, qualified

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