BeNa Culture Collection

Saccharopolyspora erythraea-BNCC
Saccharopolyspora erythraea-BNCC
  • BNCC
  • Saccharopolyspora erythraea-BNCC
  • Saccharopolyspora erythraea-BNCC

Saccharopolyspora erythraea

  • Price: $ 172
  • number:BNCC153143
  • Form:
    Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opacity Color: light yellow uplift: middle convex surface: fold texture: dry
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Saccharopolyspora erythraea
Culture medium Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate  evenly. (5) put the plates under the growth conditions.
Growth conditions 28 ℃,5-7 days, aerobic
Storage conditions 2-8 ℃
morphology Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opacity Color: light yellow uplift: middle convex surface: fold texture: dry
application Type strain; Produces erythromycins A, B
Sharing mode Public welfare sharing

Saccharopolyspora erythraea

Storage conditions : 2~8 ℃

No. : 153143

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, safety cabinet operation

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:  28 ℃, aerobic, Streptomyces medium No. 2, 5-7 days. Streptomyces medium No. 2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.  

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 5-7days. 

                                                        

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability: good viability, in 5days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)

Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: Light Yellow Protrusion: Middle Raised Surface: Wrinkled Texture: Dry

Conclusion good viability, no abnormal colony morphology, qualified

 

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