Saccharopolyspora spinosa|BNCC153056 |BNCC

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Saccharopolyspora spinosa

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃,48-72h, aerobic
Storage conditions	2-8 ℃
morphology	Colony diameter is 1-2mm, round, opaque, irregular edge, gray-white front, gray-white back, convex middle, rough surface, wrinkled surface, moist texture, easy to stir, G + (blue-purple), bacilli, purity: pure
application	Type strain
Sharing mode	Public welfare sharing

Saccharomyces spinoides

Storage conditions : 2~8 ℃

No. : 153056

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, safety cabinet operation

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:

28 ℃, aerobic, Streptomyces medium No. 2, 2-3d. Streptomyces medium No. 2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g, pH 7.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 3-5days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 2days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Untidy Transparency: Opaque Color: Light yellow Bulge: Flat Surface: Rough and wrinkled Texture: Dry and hard to pick
Conclusion:	good viability, no abnormal colony morphology, qualified

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