BeNa Culture Collection

Armillaria mellea-BNCC
Armillaria mellea-BNCC
  • BNCC
  • Armillaria mellea-BNCC
  • Armillaria mellea-BNCC

Armillaria mellea

  • Price: $ 172
  • number:BNCC143144
  • Form:
    Migratory growth, darker color, dark color
Standard strain Quantitative strain DNA extraction
Package:
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Armillaria mellea
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
morphology Migratory growth, darker color, dark color
application Eat, produce Gastrodia elata
Sharing mode Public welfare sharing

Armillaria mellea

No.: 143144

Storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: growing culture in 30 days

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4.7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates; 

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; 

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; 

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                          

item test results
viability: good viability, in 5-7 days plate colony is obvious
colony morphology:

small filamentous fungi have obvious colonies on comprehensive PDA medium, with honey yellow in the center of the colony,

the edge hyphae are white, rusty brown is sparse and flat when old, and the back of the culture medium is black.

conclusion: good viability, no abnormal colony morphology, qualified

 

Download certificate
Popular Products
Please set your password: