Lactobacillus acidophilus (Moro) Hansen and Mocquot|BNCC360065 |BNCC

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Lactobacillus acidophilus (Moro) Hansen and Mocquot

Culture medium	MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37°C,10% CO2,18-24h
Storage conditions	-20 ℃
morphology	Round, neat edges, opaque, gray-white on the front, light color, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G + (blue-purple), bacilli, purity: pure
Separation substrate	Human
application	Produces hydrogen peroxide
Sharing mode	Public welfare sharing

Genomic DNA

Product content: 5μg ;

Purity ：A260/A280 1.7-2.0

Product format: Freezed-dried

Storage conditions: 2-8 ℃

Validity period:6 months

Biosafety level: 1

Receiving notice: if any abnormality is found on the day of receipt, please contact customer service within 24 hours; after opening, it should be used up once and cannot be stored. If it is not opened temporarily, it can be stored at -20°C. Please operate in strict accordance with this instruction, otherwise it will cause abnormality, inactivation, etc., and will not provide reissue service

Handling producure:

1. Prepare sterile ddH₂O;

2. Centrifuge the sample tube 12000r /min for 1min, and add the corresponding volume of sterile ddH₂O as required;

3. It can be used normally after a short period of centrifugation after oscillation;

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