C43(DE3)pLysS Chemically Competent Cell|BNCC356373 |BNCC

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C43(DE3)pLysS Chemically Competent Cell

Culture medium	LB +10 μg/ml chloramphenicol: LB +10 μg/ml chloramphenicol: yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. After sterilization, chloramphenicol was added at a concentration of 10 μg/ml when the culture medium was cooled to 40-50 ℃.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
application	Efficient expression of toxic or hydrophobic proteins.
Sharing mode	Public welfare sharing