BeNa Culture Collection

  • TG1 Escherichia coli-BNCC

TG1 Escherichia coli

  • Price: $100
  • number:BNCC356025
  • Packing:
    Round, irregular edges, opaque, light color, light yellow on the front, light yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to pick up, G-(red), bacilli, purity: pure
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Related Products
TG1 Escherichia coli
Culture medium Base medium (LB): yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Round, irregular edges, opaque, light color, light yellow on the front, light yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to pick up, G-(red), bacilli, purity: pure
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