Enterobacter aerogenes nucleic acid detection kit (fluorescent PCR method)|BNCC361454 |BNCC

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Enterobacter aerogenes nucleic acid detection kit (fluorescent PCR method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Experimental preparation: before the experiment, sample enrichment culture was carried out according to GB 4789.41-2016; All kinds of components in the kit should be dissolved on ice before use, slightly mixed and temporarily centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15.3 μL, template 1 μL, sterile ddH2O8.7μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 57.4 ℃, 30s, 40cycle; Fluorescence channel: VIC.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	1
Separation substrate	Sputum, South Carolina Dept. of Health and Environmental Control
application	Mainly used to detect the detection of Enterobacter aerogenes in water samples of feces, suspected contaminated water, food and other samples
Sharing mode	Public welfare sharing

Certificate of Enterobacter aerogenes nucleic acid detection kit

1. Product information

Sample name: Enterobacter aerogenes nucleic acid detection kit (fluorescence PCR method)

Sample number: 361454

Sample batch: 2200505

2. Product features

Description	1.5mL cryopreservation tube package, containing reaction solution, positive quality control material, negative quality control material, DEP water
Traceability	BNCC263676(ATCC24433) staphylococcus aureus
Product specifications	1 tube of reaction solution, 1 tube of positive quality control product, 1 tube of negative quality control product, 1 tube of ddH2O water
Field of application	It is suitable for the detection of Enterobacter aerogenes in water samples such as feces, suspected contaminated water, food, etc.
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instructions

Experiment preparation: Carry out enrichment culture of samples according to GB 4789.41-2016 before the experiment; dissolve various components in the kit on ice before use, mix lightly and centrifuge briefly and place on ice for later use; Reagent preparation: experimental reaction System (25 μL), 15.3 μL of qPCR mixed reaction solution, 1 μL of template, 8.7 μL of sterile ddH2O. Reaction conditions: 95°C for 1min 1cycle; 95°C for 10s, 57.4°C for 30s for 40cycles; Fluorescence channel: VIC.

4. Notes

1. The kit should be stored at low temperature in the dark, and the various components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid the generation of air bubbles;

2. Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;

3. Judgment of results: If the Ct value of the test sample is ≥38 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 35, and two typical amplification curves appear, it is judged as positive; If the Ct value of the test sample is >35 and <38, it is recommended that the sample be redone.

5. Preservation and transportation

long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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