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Staphylococcus aureus nucleic acid detection kit certificate
1. Product information
Sample name: Staphylococcus aureus nucleic acid detection kit (multiplex fluorescence PCR method)
Sample number: 361200
Sample batch: 220402
2. Product features
3.Instructions
Experiment preparation: Before the experiment, refer to GB4789.10 or GB7918.5 for enrichment culture of samples; dissolve various components in the kit on ice before use, mix gently and centrifuge briefly and place on ice for later use; reagent preparation : Experimental reaction system (25 μL), 18.5 μL of qPCR mixed reaction solution, 1 μL of template, 5.5 μL of sterile ddH2O. Reaction conditions: 95℃ for 1min 1cycle; 95℃ for 10s, 51℃ for 30s for 40cycles; Fluorescence channel: FAM, VIC.
4. Notes
1. The kit should be stored at low temperature in the dark, and the various components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid the generation of air bubbles;
2. Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;
3. Judgment of results: if the Ct value of the test sample is ≥38 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 35, and there are two typical amplification curves, it is judged as positive; If the Ct value of the test sample is >35 and <38, it is recommended that the sample be redone.
5. Preservation and transportation
Long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.
Henan Engineering Technology Research Center of Industrial Microbial Strain
website: www.bncc.org.cn tel: 400-6699-833