Chicken-derived nucleic acid detection kit (fluorescent PCR method)|BNCC361134 |BNCC

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Chicken-derived nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 50 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescent channel: Cy5.
Storage conditions	null
Safety level	1
application	It is suitable for the identification and detection of chicken-derived ingredients in food, cosmetics and feed.
Sharing mode	Public welfare sharing

Chicken-derived nucleic acid detection kit certificate

1. Product information

Sample name: Chicken-derived nucleic acid detection kit (fluorescence PCR method)

Sample number: 361134

Sample batch: 220329

2. Product features

Reagent composition	1 tube (600μL) of qPCR mixed reaction solution; 1 tube (25μL) for positive quality control; 1 tube (25μL) for negative quality control
Packing specifications	25 T/box	Sensitivity	400copies/μL
Field of application	Nucleic acid detection for chicken-derived ingredients in food, cosmetics and feed
Save and transport	-20 ℃, valid for 1 year, dry ice transportation
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instruction

Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, and then place on ice for later use. 25μL of experimental system: 24μL of qPCR mixed reaction solution, 1μL of template; recommended reaction conditions: the first step is 95℃ for 1min, the second step is 95℃ for 10s, the third step is 50℃ for 30s, and the second to third steps are cycled 40 times; fluorescence channel select Cy5.

4.Result judgment

Negative: no exponential growth curve, or low growth curve and Ct value>35; Positive: obvious exponential growth curve, and Ct value ≤35;

5. Notes

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly, and then place on ice for use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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