Candida tropicalis nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361603 |BNCC

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Candida tropicalis nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.
Subculture procedure	This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it.
Growth conditions	Culture temperature 28 ℃; Culture time 48-72 days; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	1
morphology	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: milky white bulge: middle bulge surface: rough fold texture: easy to stir up
application	Pathogenic nucleic acid detection kit cross-reaction, specificity test, and other nucleic acid related studies.
Sharing mode	Public welfare sharing

Candida tropicalis nucleic acid reference certificate

This reference product was developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.

1. Product information

product name		Candida tropicalis nucleic acid reference (heat inactivated) (strong positive)
number	BNCC 361603	batch	220518

2. Product features

description	there are 6 pieces of this product, each containing 0.5mL, frozen
species	Fungal kingdom, Candida, Candida tropicalis
traceability	Henan province industrial microbial strain engineering technology research center & larr;CGMCC 2.587
preparation method	inoculation of broth medium, centrifugal collection, PBS suspension, 80 ℃ water bath inactivation for 1 hour
other ingredients	PBS, stabilizer
store	-80 ℃ valid for 2 years
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Quality inspection

project	standard	confirm test results
bacterial concentration	≥1.0×10⁷CFU/mL	5.7×10⁷CFU/mL
authentication	phenotypic characteristics, biochemical identification	Exactly consistent with Candida tropicalis
authentication	gene sequencing	similarity rate 100%

4. Instructions

This reference product is not recommended for repeated freezing and thawing. Before use, take it out of the refrigerator, equilibrate to room temperature, mix well, and it is ready to use.

5. Notes

1. After receiving the goods, if any damage is found, please contact the center in time within 24 hours, and reissue it free of charge after confirmation;

2. This product uses heat inactivation method, which is likely to cause protein damage, and is only suitable for nucleic acid and other tests;

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833

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