Bordetella parapertussis nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361600 |BNCC

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Bordetella parapertussis nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	37 ℃,24-48h, aerobic
Storage conditions	-80 ℃
Safety level	2
application	Research on Reagents for Detection of Pathogen Nucleic Acid
Sharing mode	Public welfare sharing

Certificate of nucleic acid reference for bacilli paratoticus

1. Product information

Sample name: pertussis paracarbacillus nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361600

Sample batch: 220627

2. Product features

description	1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, is transparent liquid
traceability	ATCC 53892 pertussis paratussis
preparation method	inoculation of BHI + 5% FBS liquid medium, centrifugal collection, PBS suspension, 80 ℃ water bath inactivation for 1h
concentration value	≥1.0×10⁸CFU/mL	reference value	1.74×10⁹CFU/mL
applicable method	related research suitable for pathogen nucleic acid detection reagents
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be treated according to infectious substances. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage: -80℃, valid for 2 years; transport conditions: transport on dry ice.

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833

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