Clostridium difficile nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361598 |BNCC

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Clostridium difficile nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃; Culture time 24~48 hours; Anaerobic gas environment;
Storage conditions	-80 ℃
Safety level	0
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of Clostridium difficile nucleic acid detection reagent for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Clostridium difficile nucleic acid reference certificate

1. Product information

Sample name: Clostridium difficile nucleic acid reference certificate (heat inactivated) (strong positive)

Sample number: 361598

Sample batch: 220523

2. Product features

Description	Packaged in 1.5mL cryovials, containing 0.5mL nucleic acid reference, in liquid state
Traceability	ATCC 43593-FZ Clostridium difficile
Preparation method	Inoculated with Columbia blood plate medium, eluted with PBS, and inactivated in a water bath at 80°C for 30min
concentration standard	≥10⁷CFU/mL	Reference value	1.7×10⁷CFU/mL
authentication	Colony morphology consistent with C. difficile	results	match
*Note: If you have any questions about the reference materials, please contact our center (BNCC) for assistance before use

3. Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4.Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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