Escherichia coli ETEC nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361518 |BNCC

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Escherichia coli ETEC nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Tryptone soybean medium (English name: TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Storage conditions	-80 ℃, kept away from light
Safety level	2
Separation substrate	Fecal specimens from diarrhea cases
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product is an E. coli (enterotoxigenic) nucleic acid detection reagent evaluation with heat inactivation strong positive reference material for early diagnosis of infection And quality control.
Sharing mode	Public welfare sharing

E. coli (enterotoxigenic) nucleic acid reference certificate

1. Product information

Sample name: Escherichia coli (enterotoxigenic) nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361518

sample batch: 220511

2. Product features

description	Packaged in a 1.5mL cryovial containing 0.5mL nucleic acid reference material in a transparent liquid state
traceability	CICC10667 enterotoxigenic Escherichia coli
Preparation method	Inoculated tryptone soybean broth medium, collected by centrifugation, suspended in PBS, and inactivated in a water bath at 80 °C for 1 h
Concentration value	≥1.0×10⁸CFU/mL	Reference value	4.6×10⁸CFU/mL
applicable method	Related research on nucleic acid detection reagents for pathogens
*Note: If you have any questions about the reference materials, please contact our center (BNCC) for assistance before use

3. Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4.Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Storage and transportation

Long-term storage: -20℃, valid for 2 years; transport conditions: transport on dry ice.

Henan Engineering Technology Research Center of Industrial Microbial Strain

Website: www.bncc.org.cn Tel: 400-6699-833

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