Citrobacter freundii (Braak) Werkman and Gillen nucleic acid standard quality control product|BNCC361460 |BNCC

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Citrobacter freundii (Braak) Werkman and Gillen nucleic acid standard quality control product

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, the nucleic acid reference is dissolved on ice, slightly mixed and briefly centrifuged and placed on ice for later use.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	1
application	It is used for the confirmation and evaluation of relevant measurement methods and the quality control of the analysis process in the experimental analysis of the nucleic acid detection of Citrobacter freuntilii.
Sharing mode	Public welfare sharing

Citrobacter freund nucleic acid standard quality control certificate

1. Product information

Sample name: Citrobacter freund nucleic acid standard quality control

Sample number: 361460

Sample batch: 220505

2. Product features

Reagent composition	1.5mL frozen storage tube package, containing 100μL nucleic acid quality control product, transparent liquid state; Positive quality control 1 tube (25μL); Negative quality control 1 tube (25μL)
traceability	ATCC43864
packing specifications	100μL/branch
fixed value method	qPCR
reference value	10⁵~10⁶copies/μL
applicable fields	It is suitable for confirmation and evaluation of relevant measurement methods and quality control in the nucleic acid detection and analysis of Citrobacter freunii.
save and transport	-20 ℃, valid for 1 year, dry ice transportation
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Instruction

Before use, dissolve the nucleic acid reference material on ice, mix gently and centrifuge briefly, then place on ice for later use

4. Notes

1. After receiving the goods, please keep it properly. if it is damaged, please contact the center within 24 hours after receiving it.

2. This nucleic acid quality control product should be used as soon as possible after freezing and thawing. it can be kept at -20 ℃ and below for a long time, but repeated freezing and thawing for more than 3 times should be avoided.

3. Sample treatment, reagent preparation and sample addition should be carried out in different areas to avoid cross contamination.

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833

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