Streptococcus pneumoniae nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361401 |BNCC

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Streptococcus pneumoniae nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Brain heart extract medium (English name: BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Growth conditions	37 ℃,18-24h,5% CO2 + 95% air
Storage conditions	-80 ℃
Safety level	2
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of Streptococcus pneumoniae nucleic acid detection reagent for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Streptococcus pneumoniae nucleic acid reference certificate

1. Product information

Sample name: Streptococcus pneumoniae nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361401

Sample batch: 220509

2. Product features

Description	Packaged in a 1.5mL cryovial, containing 0.5mL nucleic acid reference material, in liquid state
Traceability	ATCC33152 Legionella pneumophila
Preparation method	Inoculated on BCYE agar medium, eluted with PBS, and inactivated in a water bath at 80°C for 1h
Concentration standard	≥1.0×10⁷CFU/mL	results	4.9×10⁷CFU/mL
Authentication	Colony morphology consistent with Streptococcus pneumoniae	results	match
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference material at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. Nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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