Filobasidiella bacillispora nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361399 |BNCC

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Filobasidiella bacillispora nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	YM medium (English name: YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Storage conditions	-80 ℃
Safety level	1
application	This product is a strongly positive reference for heat inactivation for evaluation of Cryptococcus neoformans nucleic acid detection reagent.
Sharing mode	Public welfare sharing

Cryptococcus neoformans nucleic acid reference certificate

1. Product information

Sample name: Cryptococcus neoformans nucleic acid reference material (heat inactivated) (strong positive)

Sample number: 361399

Sample batch: 220503

2. Product features

Description	Packaged in a 1.5mL cryovial, containing 0.5mL nucleic acid reference material, in liquid state
Traceability	Cryptococcus neoformans ATCC32609
Preparation method	Inoculate yeast extract wort medium, wash with PBS, inactivate in 80°C water bath for 1h
Concentration standard	≥1.0×10⁷CFU/mL	results	5.4×10⁷CFU/mL
Authentication	The colony morphology is consistent with Cryptococcus neoformans	results	match
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference material at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. Nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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