Quality control sample of total number of colonies in feed|BNCC361567 |BNCC

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Quality control sample of total number of colonies in feed

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	After taking out the quality control sample from the refrigerator at -20 ℃, placing it at room temperature for 30 min, then opening it in the biological safety cabinet, immediately adding 1.1mL of sterile water or sterile physiological saline to re-dissolve the freeze-dried quality control product, and fully mixing it to prepare a sample suspension, which is a quality control sample with a concentration of 2.0~2.6 × 10 ^ 6CFU/ml.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-20 ℃
Safety level	2
application	GB/T 13093-2006 Determination of the total number of bacteria in feed, etc.
Sharing mode	Public welfare sharing

Quality control sample certificate for the total number of feed bacterial colonies

1. Product information:

sample name: Quality control sample of total number of feed colonies

Sample number: 361567

Sample batch: 220516

2. Product features:

description	Appearance is white powder (lyophilized powder), containing protective agent, packaged in 7mL vial.
traceability	ATCC 25922 Escherichia coli, ATCC 6633 Bacillus subtilis, ATCC 6538 Staphylococcus aureus
product specifications	2.0~2.6×10⁶CFU/ml
Reference value	2.3×10⁶CFU/ml
applicable method	GB/T 13093-2006 Determination of total bacteria in feed, etc.
*Note: If you have any questions about the product, please contact our center (BNCC) for help before use.

3. Instructions

After taking the quality control samples out of the -20°C refrigerator, they were placed at room temperature for 30 minutes to equilibrate, and then opened in the biological safety cabinet. Immediately add 1.1 mL of sterile water or sterile normal saline to reconstitute the lyophilized quality control samples, and fully reconstitute the lyophilized samples. After mixing, prepare a sample suspension, which is the quality control sample, with a concentration of 2.0~2.6× 10⁶CFU/ml.

4.Notes

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.

2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.

3. This product is a live microbial product, which has certain risks of biological safety. Please do not directly touch the sample in the bottle with your hands. After use, please sterilize the residual sample at high temperature and high pressure and discard it.

5. Preservation and transportation

long-term storage:-20 ℃, valid for 1 year; Transportation conditions: ice bag transportation, the time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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